Evaluation of the GENE-UP® Salmonella 2 Assay for Detecting Salmonella in Ground Beef: An AOAC OMA Collaborative Study
N. J. Taylor )
Salmonella causes an estimated 1.4 million cases of foodborne illness and over 400 deaths every year in the United States alone. As a result, the USDA-FSIS have recently proposed the tightening of the performance standards in identifying Salmonella spp. in ground beef and beef trimmings. It is critical, therefore, that the most robust and accurate analytical methods are available to detect low levels of Salmonella spp. The objective of this study was to statistically compare the method performance of the candidate method, GENE-UP Salmonella 2, against the reference method, USDA FSIS MLG 4.01. Results from this multi-laboratory collaborative study indicated that the GENE-UP Salmonella 2 method is a robust Real-time PCR method for the rapid detection of Salmonella spp. in raw ground beef. An Expert Review Panel formed within the Association of Official Analytical Chemists (AOAC) granted Official Methods of Analysis℠ (OMA) First Action status to the GENE-UP Salmonella 2 assay (OMA certification number 2020.02). This designation will have a significant effect on increased market acceptance and integration of the method into routine use for beef producers.
NOTE: This study has been published in in the Journal of AOAC International and can be viewed by following this link: https://doi.org/10.1093/jaoacint/qsab005